A Comparative Study on Mechanical and Biochemical Properties of Bovine Pericardium After Single or Double Crosslinking Treatment
نویسندگان
چکیده
BACKGROUND AND OBJECTIVES Glutaraldehyde (GA) has been used as a representative method of tissue preservation in cardiovascular surgery. However, GA has showed limited durability including calcification, mechanical failure and toxicity. To overcome those unsolved problems, we analyzed the crosslinking differences of primary amines, GA and genipin in their mechanical and biochemical properties with a single or double crosslinking agent for clinical application. MATERIALS AND METHODS Samples were divided into 3 groups; control, single crosslinking fixation and double crosslinking fixation after decellurarization using bovine pericardium. For analysis of the biochemical and mechanical properties of each crosslinking method, tensile strength, percentage strain, thermal stability, resistance to pronase, nynhydrin and cytotoxicity test were studied. RESULTS Combined hexamethylene diamine and suberic acid in the carbodiimide hydrochloride/N-hydroxysucinimide solution (EDC/NHS) after decellurarization, tensile strength and strain percentage were not statistically significant compared to the single crosslinking treated groups (p>0.05). Tissue crosslinking stability was weak in single treatment of diphenylphosphoryl azide, suberic acid, low concentration of EDC, hexamethylene diamine and procyanidin groups, but thermal stability and resistance to the pronase and ninhydrin were markedly increased in concentrated EDC/NHS or after combined double treatment with low concentration of GA or genipin (p<0.001). CONCLUSION Single or double crosslinking with low concentration of carbodiimide, diphenylphosphonyl azide, procyanidin, suberic acid and hexane diamine were not as effective in mechanical, biochemical, cytotoxic and crosslinking properties compared to GA or genipin fixation, but their mechanical and chemical properties were much improved when combined with low concentrations of GA or genipin in the double crosslinking process.
منابع مشابه
A Comparative Study of Multi-Attribute Continuous Double Auction Mechanisms
Auctions have been as a competitive method of buying and selling valuable or rare items for a long time. Single-sided auctions in which participants negotiate on a single attribute (e.g. price) are very popular. Double auctions and negotiation on multiple attributes create more advantages compared to single-sided and single-attribute auctions. Nonetheless, this adds the complexity of the auctio...
متن کاملComparative evaluation of glycerolized bovine pericardium implant with prolene mesh for closure of large abdominal wall defects in dogs
The aim of this investigation was to evaluate the use of polypropylene mesh and glycerol preserved bovine pericardium (GBP) to repaire abdominal wall defects in 24 clinically healthy mongrel dogs using inlay and underlay techniques. Twenty four healthy mongrel dogs were used in this study. The animals were divided into two main groups according to the type of the prosthetic material used. Each ...
متن کاملChanges of the Structural and Biomechanical Properties of the Bovine Pericardium after the Removal of α-Gal Epitopes by Decellularization and α-Galactosidase Treatment
BACKGROUND Bovine pericardium is one of the most widely used materials in bioprosthetic heart valves. Immunologic responses have been implicated as potential causes of limited durability of xenogenic valves. This study aimed to determine the effectiveness of decellularization and α-galactosidase (α-gal) to remove major xenoreactive antigens from xenogenic tissues. MATERIALS AND METHODS Recomb...
متن کاملA Comparative Study on the Microstructure and Mechanical Properties of Al-Si-Cu/1wt %NCP Composites after T6 Heat Treatment
In this article, microstructural characteristics and mechanical properties of Al-Si-Cu/NCP composites were evaluated. Reinforced nanocomposites with 1 wt% nano-clay were fabricated by the method of the stir casting. Stirring times and temperatures were variable parameters to produce specimens. Consequently, the effect of a T6 heat treatment, which contained solutioning at 490 ºC for ...
متن کاملComparison between the decellularized bovine pericardium and the conventional bovine pericardium used in the manufacture of cardiac bioprostheses
Introduction: In this paper, our objective was to compare the decellularized and conventional pericardium mechanical resistance and also its capability of inducing inflammatory response in an animal experimental model. Methods: In order to study these properties, we divided the pericardia into two groups: Group I pericardium conventionally treated with GTA and Group II – pericardium previously ...
متن کامل